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> Anti-Nucleoprotein (N) of Novel Coronavirus SARS-CoV-2/ 2019-nCoV (capture antibody)

Anti-Nucleoprotein (N) of Novel Coronavirus SARS-CoV-2/ 2019-nCoV (capture antibody)

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Referência AS214576

Tamanho : 100ul

Marca : Agrisera

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Anti-Nucleoprotein (N) of Novel Coronavirus SARS-CoV-2/ 2019-nCoV (capture antibody)

Product no: AS21 4576

AS21 4576   | Clonality: Monoclonal |  Host: Mouse | Reactivity: Nucleaoprotein (N) of Novel Coronavirus SARS-CoV-2/ 2019-nCoV (human)
(Capture antibody)

    Product Info
    Sub class: IgG1
    Immunogen: Recombinant Human Novel Coronavirus Nucleoprotein (N) (1-419aa), UniProt: P0DTC9
    Host: Mouse
    Clonality: Monoclonal
    Purity: Affinity chrompatography purified in 10 mM PBS, pH 7.4, 50 % glycerol, 0.03% Proclin 300.
    Format: Liquid
    Quantity: 100 µl
    Storage: Store at -20°C or -80°C. Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: ELISA (ELISA), Western blot (WB)
    Recommended dilution: 1: 1000 - 1: 5000 (ELISA), 1: 1000-1: 5000 (WB)
    Expected | apparent MW: 48 | 55 kDa
    Reactivity
    Confirmed reactivity: Human Nucleoprotein (N) of Novel Coronavirus SARS-CoV-2/ 2019-nCoV
    Application Examples



    Recombinant  SARS-CoV-2 nucleocapsid recombinant protein overexpressed in E. coli, was separated on SDS-PAGE and transferred to a PVDF membrane (pore size 0.45 µm). Following the blocking with 5 % non-fat milk in PBS-T, primary anti- Nucleoprotein (N) antibodies were used in 1: 1000 in 5 % non-fat milk in PBS-T, followed by washes and incubation with a secondary antibody goat anti-mouse IgG. Reaction was visualized using chemiluminescence.

    ELISA confirming the binding activity of SARS-CoV-2-N antibodies in a functional ELISA.
    Human Novel Coronavirus Nucleoprotein(N) (AS20 4388) was immobilized at 2 μg/ml and can bind SARS-CoV-2-N antibody, the EC50 is 27.78 to 41.57 ng/ml.
    Additional Information
    Additional information: This product is a capture antibody, which can be combined with Detection antibody: 
    AS21 4577 | Anti-Nucleoprotein (N) of Novel Coronavirus SARS-CoV-2/ 2019-nCoV (human), monoclonal antibodiesand Positive control: 
    AS20 4388 | Human Novel Coronavirus Nucleoprotein(N)
    Background
    Background: Nucleaoprotein (N) of Novel Coronavirus SARS-CoV-2/ 2019-nCoV (human) plays a fundamental role during virion assembly through packaging of the positive strand viral genome RNA into a helical ribonucleocapsid (RNP).

    Alternative names:     NC, Protein N, Nucleocapsid protein
    Protocols Antibody protocols Reviews:

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