D355-25 | Anti-Human Desmoglein-1 (Clone 129204) Quimigen Portugal

AntiHuman Desmoglein1 [Clone 129204]

Product No.: D355


Clone 129204 Target Desmoglein1 Formats AvailableView All Product Type Monoclonal Antibody Alternate Names CDHF4, DG1, DSG Isotype IgG2a Applications ELISA Indirect , ICC , WB


Antibody Details Product Details Reactive Species Human Host Species Mouse Immunogen NS0Derived Recombinant Human Desmoglein1 Extracellular Domain (Accession # Q02413) Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 7.4 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present. Storage and Handling The lyophilized antibody can be stored desiccated at 20°C to 70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 28°C. For longterm storage of the reconstituted antibody, aseptically aliquot into working volumes and store at 20°C to 70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2829935 Applications and Recommended Usage? Quality Tested by Leinco Indirect ELISA: This antibody can be used in an indirect detection ELISA at 0.5 1.0 µg/ml with a suitable second step reagent such as PN:M114. The detection sensitivity of this indirect ELISA for Human Desmoglein1 is approximately 1 ng/well. Western Blotting: To detect Human Desmoglein1 this monoclonal antibody can be used at a concentration of 12 µg/ml. This monoclonal antibody should be used in conjunction with compatible secondstep reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The The detection limit forrhDesmoglein1 is approximately 5 ng/lane and 0.5 ng/lane under nonreducing and reducing conditions, respectively. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. Additional Applications Reported In Literature ? Immunocytochemistry: Suitable for use at concentration of 525 µg/mL. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. Description Description Specificity Mouse AntiHuman Desmoglein1 (DSG1) (Clone 129204) recognizes an epitope on Human DSG1. This monoclonal antibody was purified using multistep affinity chromatography methods such as Protein A or G depending on the species and isotype. Background Desmoglein 1, also known as DSG1, is a human gene. Desmosomes are cellcell junctions between epithelial, myocardial and certain other cell types. Desmoglein 1 is a calciumbinding transmembrane glycoprotein component of desmosomes in vertebrate epithelial cells. Currently, three desmoglein subfamily members have been identified and all are members of the cadherin cell adhesion molecule superfamily. DGI expression may be a significant factor for invasion, metastasis, and prognosis of human esophageal cancer. ¹ PubMed Desmoglein1 NCBI Gene Bank ID 1828 UniProt.org More Information on Uniprot.org References & Citations 1. Berean, K. et al. (2008) Pathology. 40(6):6116. View product citations for antibody D355 on CiteAb This product can not be ordered from your electronic catalog. A quotation will be created for this reference, would you like to continue? In this case, make sure that you have filled in the desired quantity E-mail Rua Almada Negreiros Lote 5, Loja 14 2615-275 Alverca do Ribatejo - Portugal Telefone: +351 30 8808 050 (Chamada para a rede fixa nacional) Fax: +351 30 8808 052 (Chamada para a rede fixa nacional) Email: info@quimigen.pt Outras informações Como encomendar ? Condições de venda Ofertas de emprego Quem somos nós? Newsletter Políticas de qualidade Eventos Aviso legal Todas as marcas comerciais ou marcas registradas que aparecem neste site são de propriedade de seus respectivos proprietários. pt en es

Antibody Details

Product Details

Reactive Species

Human

Host Species

Mouse

Immunogen

NS0Derived Recombinant Human Desmoglein1 Extracellular Domain (Accession # Q02413)

Endotoxin Level

<0.1 EU/µg as determined by the LAL method

Formulation

This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 7.4 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.

Storage and Handling

The lyophilized antibody can be stored desiccated at 20°C to 70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 28°C. For longterm storage of the reconstituted antibody, aseptically aliquot into working volumes and store at 20°C to 70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.

Country of Origin

USA

Shipping

Next Day Ambient

RRIDAB_2829935

Applications and Recommended Usage?
Quality Tested by Leinco

Indirect ELISA: This antibody can be used in an indirect detection ELISA at 0.5 1.0 µg/ml with a suitable second step reagent such as PN:M114. The detection sensitivity of this indirect ELISA for Human Desmoglein1 is approximately 1 ng/well.
Western Blotting: To detect Human Desmoglein1 this monoclonal antibody can be used at a concentration of 12 µg/ml. This monoclonal antibody should be used in conjunction with compatible secondstep reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The The detection limit forrhDesmoglein1 is approximately 5 ng/lane and 0.5 ng/lane under nonreducing and reducing conditions, respectively. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.

Additional Applications Reported In Literature ?

Immunocytochemistry: Suitable for use at concentration of 525 µg/mL.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Mouse AntiHuman Desmoglein1 (DSG1) (Clone 129204) recognizes an epitope on Human DSG1. This monoclonal antibody was purified using multistep affinity chromatography methods such as Protein A or G depending on the species and isotype.

Background

Desmoglein 1, also known as DSG1, is a human gene. Desmosomes are cellcell junctions between epithelial, myocardial and certain other cell types. Desmoglein 1 is a calciumbinding transmembrane glycoprotein component of desmosomes in vertebrate epithelial cells. Currently, three desmoglein subfamily members have been identified and all are members of the cadherin cell adhesion molecule superfamily.
DGI expression may be a significant factor for invasion, metastasis, and prognosis of human esophageal cancer. ¹

PubMed

Desmoglein1

NCBI Gene Bank ID

1828

UniProt.org

More Information on Uniprot.org