Anti-Human cMyc (Clone 9E10) - HRP

Cterminal region of human cmyc

This HRPconjugated antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 7.4, 1% BSA. (Warning: Use of sodium azide as a preservative will inhibit the enzyme activity of horseradish peroxidase)

Liquid

This horseradish peroxidase conjugated monoclonal antibody is stable when stored at 28°C. Do not freeze.

Research Use Only

2 – 8° C Wet Ice

IHC,
IP,
IF,
WB,
ELISA,
FC

9E10 activity is directed against human cMyc and can crossreact with denatured murine cMyc in some circumstances.

cMyc is an essential transcription factor that belongs to the superfamily of basic helixloop helix DNAbinding proteins that function in normal embryogenesis, acquisition and maintenance of stem cell properties, metabolism, cellular division, and cell death^{1, 2}. cMyc is also a protooncogene, originally identified in Burkitt lymphoma¹. Its dysregulation, by mutation or epigenetic modification, has been observed in over 50% of human cancers³. cMyc regulates various cancer cellular functions, including cell cycle, survival, proliferation, and metabolic reprogramming¹, and is associated with tumor aggression and chemoresistance³. cMyc has been suggested as a target for cancer immunotherapy ²and various methods of inhibition have been studied^{1, 2, 3}.

9E10 was generated by immunizing a BALB/c mouse with a synthetic peptide corresponding to residues 408432 of human cMyc conjugated to keyhole limpet hemocyanin via the cysteine residues⁴. Splenocytes were fused with SP2 myeloma cells. 9E10 failed to immunoprecipitate protein from Colo 320 HSR cell extracts during screening but did detect cMyc in Western blotting. The 9E10 epitope has become a wellknown affinity tag used in recombinant protein expression, i.e., the myctag⁵. The target epitope is the C terminal EQKLISEEDL peptide with the core sequence being LISE⁵and the crystal structure and binding mode have been solved⁶. 9E10 detection has highly variable epitope recognition that is dependent on neighboring sequence context⁷. Additionally, crossreactivity of 9E10 has been observed in murine cell lines in a fluorescence assay and by Western blotting⁸.

cMyc is ubiquitously expressed during tissue development and in a wide variety of tumors. cMyc is often studied in embryonic stem cells and induced pluripotent stem cells. cMyc is mainly associated with cell nuclei.

P09416

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