Anti-Human CD27
Referência C1377-25
Tamanho : 25ug
Marca : Leinco Technologies
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AntiHuman CD27
AntiHuman CD27
Product No.: C1377
Target CD27 Product Type Polyclonal Antibody Alternate Names MGC20393, S152, T14, TNFRSF7, Tp55 Applications CyTOF® , FC , IHC , N , WB |
Antibody DetailsProduct DetailsReactive Species Human Host Species Goat Immunogen Purified Recombinant Human CD27 (>98%) Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present. State of Matter Lyophilized Storage and Handling The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at 20°C to 70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 28°C. For longterm storage of the reconstituted antibody, aseptically aliquot into working volumes and store at 20°C to 70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2828432 Applications and Recommended Usage? Quality Tested by Leinco Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing CD27. A suggested method would be to stain cells expressing CD27 with 0.25 µg per 1 x 106 cells in a total staining volume of 100 µl followed by PN:G641. Western Blotting: To detect Human CD27 this polyclonal antibody can be used at a concentration of 1 µg/ml. This polyclonal antibody should be used in conjunction with compatible secondstep reagents such as PN:G505 and a chromogenic substrate such as PN:T343. The detection limit for Human CD27 is 5 ng/lane under nonreducing and 25 ng/lane under reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:C1339. Additional Applications Reported In Literature ? Neutralization: This antibody is useful for neutralization of Human CD27 bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Human CD27 (at 3 ng/ml) is 2.259 µg/ml. Immunohistochemistry: Suitable for use at concentration of 315 µg/mL. CyTOFready: Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Goat AntiHuman CD27 recognizes Human CD27. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research. Background CD27 is a tumor necrosis factor receptor. The protein encoded by this gene is a member of the TNFreceptor superfamily. This receptor is required for generation and longterm maintenance of T cell immunity. It exists as both a disulfidelinked dimer on the cell surface and as a soluble protein found in serum. It binds to ligand CD70 which is expressed on thymic stromal cells and a small subset of activated T cells and plays a key role in regulating Bcell activation and immunoglobulin synthesis. This receptor transduces signals that lead to the activation of NFκB and MAPK8/JNK. Adaptor proteins TRAF2 and TRAF5 have been shown to mediate the signaling process of this receptor. CD27binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor. PubMed NCBI Gene Bank ID UniProt.org References & CitationsVocê também pode estar interessado nos seguintes produtos:
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