| Short Description : | Mouse monoclonal anti-Heat shock protein beta-1 for use in WB, IHC, IF, FC and ELISA in Human and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | WB/IHC/IF/FC/ELISA |
| Host: | Mouse |
| Reactivity: | Human/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Monoclonal |
| Clone ID : | 5D7 |
| Isotype: | IgG1 |
| Conjugation: | Unconjugated |
| Purification: | Affinity purification |
| Dilution Range: | WB 1:500-1:2000IHC 1:200-1:1000IF 1:200-1:1000FC 1:200-1:400ELISA 1:10000 |
| Formulation: | Liquid in PBS containing 0.03% Sodium Azide, 0.5% BSA, 50% Glycerol. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
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| Immunogen: | Purified recombinant fragment of human HSP27 expressed in E. Coli. |
| Specificity: | HSP27 Monoclonal Antibody detects endogenous levels of HSP27 protein. |
| Post Translational Modifications | Phosphorylated upon exposure to protein kinase C activators and heat shock. Phosphorylation by MAPKAPK2 and MAPKAPK3 in response to stress dissociates HSPB1 from large small heat-shock protein (sHsps) oligomers and impairs its chaperone activity and ability to protect against oxidative stress effectively. Phosphorylation by MAPKAPK5 in response to PKA stimulation induces F-actin rearrangement. |
| Function | Small heat shock protein which functions as a molecular chaperone probably maintaining denatured proteins in a folding-competent state. Plays a role in stress resistance and actin organization. Through its molecular chaperone activity may regulate numerous biological processes including the phosphorylation and the axonal transport of neurofilament proteins. |
| Protein Name | Heat Shock Protein Beta-1Hspb128 Kda Heat Shock ProteinEstrogen-Regulated 24 Kda ProteinHeat Shock 27 Kda ProteinHsp 27Heat Shock Protein Family B Member 1Stress-Responsive Protein 27Srp27 |
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| Cellular Localisation | CytoplasmNucleusCytoskeletonSpindleCytoplasmic In Interphase CellsColocalizes With Mitotic Spindles In Mitotic CellsTranslocates To The Nucleus During Heat Shock And Resides In Sub-Nuclear Structures Known As Sc35 Speckles Or Nuclear Splicing Speckles |
| Alternative Antibody Names | Anti-Heat Shock Protein Beta-1 antibodyAnti-Hspb1 antibodyAnti-28 Kda Heat Shock Protein antibodyAnti-Estrogen-Regulated 24 Kda Protein antibodyAnti-Heat Shock 27 Kda Protein antibodyAnti-Hsp 27 antibodyAnti-Heat Shock Protein Family B Member 1 antibodyAnti-Stress-Responsive Protein 27 antibodyAnti-Srp27 antibodyAnti-HSPB1 antibodyAnti-HSP27 antibodyAnti-HSP28 antibody |
Information sourced from Uniprot.org