Anti-H3K4me1T6p | Histone H3 (monomethylated Lys4, p Thr6)
Referência AS163171
Tamanho : 50ug
Marca : Agrisera
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Anti-H3K4me1T6p | Histone H3 (monomethylated Lys4, p Thr6)
Product no: AS16 3171
AS16 3171 | Clonality: Polyclonal | Host: Rabbit | Reactivity: C.elegans, D. melanogaster, Human, Mouse, plant, Rat, Xenopus sp.
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| Immunogen: | KLH-conjugated synthetic peptide |
| Host: | Rabbit |
| Clonality: | Polyclonal |
| Purity: | Immunogen affinity purified serum. |
| Format: | Liquid |
| Quantity: | 50 µg |
| Storage: | Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. |
| Tested applications: | Chromatin immunoprecipitation (ChIP), Dot blot (Dot), Immunofluorescence (IF), Immunohistochemistry (IHC), Western blot (WB) |
| Recommended dilution: | 2-5 µg/million cells (ChIP), 1 : 1000 (Dot), 1 : 50 (IF), 1 : 50 (IHC), 1 : 500 (WB) |
| Expected | apparent MW: | 15 kDa |
| Confirmed reactivity: | Human |
| Predicted reactivity: | Caenorhabditis elegans, Drosophila melanogaster, Mouse, Plant, Rat, Xenopus sp. |
| Not reactive in: | No confirmed exceptions from predicted reactivity are currently known |
application example Dot blot using anti-H3K4me1T6p antibodies. Load: 1, 10, and 100 picomoles of peptide. Primary antibody used at a 1:1 000 dilution for 45 min at 4°C. Secondary antibody: Dylight®488 rabbit secondary antibody at 1:10 000 for 45 min at RT.  Immunofluorescence using anti- H3K4me1T6p antibodies. Tissue: HeLa cells. Fixation: 0.5% PFA. Primary antibody used at a 1:50 dilution for 1 h at RT. Secondary antibody: FITC secondary antibody at 1:10 000 for 45 min at RT. Localization: Histone H3K4me1T6p is nuclear and chromosomal. Staining: H3K4me1T6p is expressed in green and the nuclei are counterstained with DAPI (blue).  Western Blot using anti-Rabbit H3K4me1T6p antibodies. 30 μg of C. elegans embryonic lysate. Primary antibody used at a 1:500 dilution overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10 000 for 45 min at RT.  Western Blot using anti-H3K4me1T6p antibodies. 30 μg of HeLa histone extracts per lane. Primary antibody used at a 1:500 dilution overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10 000 for 45 min at RT. |
| Additional information: | This antibody preparation is provided in 20 mM Potassium Phosphate pH 7,2, 150 mM NaCl, 0,01% sodium azide and 30% glycerol |
| Background: | Methylation of lysine 4 on H3 (H3K4Me) and phosphorylation of threonine 3 (H3T3p) are known marks of transcriptional activation and mitosis. H3K4 has many known modifying enzymes (Set1, Set7/9, MLL, ASH1). |