75-149 | Anti-GABA-A-R, Beta3 Antibody Quimigen Portugal

SKU: 75-149

Ships: 1-2 business days

Product Details

Target

GABA(A)R, Beta3

Target Description

Gamma-aminobutyric acid receptor subunit beta-3 or GABA AR-Beta3 is encoded by the gene GABRB3. GABAA receptors are members of the Cys-loop family of ligand-gated ion channels. GABA AR-beta3 a is a subunit of the pentameric GABA receptors where GABA is the major inhibitory neurotransmitter in the vertebrate brain and mediates neuronal inhibition by binding to the GABA receptor and opening an integral chloride channel. GABA AR beta3 has been found to be expressed in retina, heart and primarily in brain at the cell junction, synapse, postsynaptic cell membrane and cytoplasmic vesicle membrane. This gene may be associated with the pathogenesis of several disorders including epilepsy and autism, childhood absence epilepsy 5 and early infantile epileptic encephalopathy 43.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N87/25

Isotype

IgG1

Applications

ICC, IHC, IP, WB

Host Species

Mouse

Gene Name

Gabrb3 Gabrb-3

Molecular Weight

55 kDa

Antigen

Fusion protein amino acids 370-433 of mouse GABA-A-R-Beta3 (accession number AAB60502) produced recombinantly in E. Coli

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_2109585

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.76

Dilution Ranges

WB: 1:1000
IHC: 1:500
ICC: 1:500
IP: 2 ug

Conjugate

Unconjugated

Specificity

No cross-reactivity against GABA-A-R-Beta1 or –Beta2

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Shipping

Shipped on ice packs

Synonyms

Gamma-aminobutyric acid receptor subunit beta-3 (GABA(A) receptor subunit beta-3)

UniProt ID

P63080

Citations (45)

Product Specific References for Applications and Species

Immunocytochemistry: Human | Mouse | Rat
Immunohistochemistry: Mouse | Rat
Immunoprecipitation: Rat
Western Blot: Human | Mouse | Rat
Additional Publications: Unspecified

Immunocytochemistry: Human
PMID	Dilution	Publication
23236276	1:250	Fuks, J.M., et al. 2012. GABAergic signaling is linked to a hypermigratory phenotype in dendritic cells infected by Toxoplasma gondii.. PLoS Pathogens, e1003051.

Immunocytochemistry: Mouse
PMID	Dilution	Publication
39357522	1:200	Wang, G, et al. 2024. The TMEM132B-GABAA receptor complex controls alcohol actions in the brain. Cell, .
36997609	1:300	Giansante, G., et al. 2023. Neuronal Network Activity and Connectivity are Impaired in a Conditional Knockout Mouse Model with PCDH19 Mosaic Expression. Molecular Psychiatry, .
34936876	1:1000	Garcia, J.D., et al. 2021. Stepwise disassembly of GABAergic synapses during pathogenic excitotoxicity. Cell Reports, 110142.
31835170	1:500	Niwa, F., et al. 2019. cAMP-EPAC-Dependent Regulation of Gephyrin Phosphorylation and GABAAR Trapping at Inhibitory Synapses. iScience, 453-465.
31665629	1:200	Moretto, E., et al. 2019. TSPAN5 Enriched Microdomains Provide a Platform for Dendritic Spine Maturation through Neuroligin-1 Clustering. Cell Reports, 1130-1146.e8.
31315046	not listed	Nathanson, A.J., et al. 2019. Identification of a Core Amino Acid Motif within the α Subunit of GABAARs that Promotes Inhibitory Synaptogenesis and Resilience to Seizures. Cell Reports, 670-681.
30667360	1:5 (supe)	Andrews, N.P., et al. 2019. A toolbox of IgG subclass-switched recombinant monoclonal antibodies for enhanced multiplex immunolabeling of brain. Elife, e43322.
23672680	1:250	Alicea, B., et al. 2013. Defining the diversity of phenotypic respecification using multiple cell lines and reprogramming regimens.. Stem Cells and Development, 2641-2654.
21735059	1:500	Yanovsky, Y., et al. 2012. GABAA receptors involved in sleep and anaesthesia: β1- versus β3-containing assemblies.. Pflügers Archiv: European Journal of Physiology, 187-199.

Immunocytochemistry: Rat
PMID	Dilution	Publication
34047007	1:500	Yang, X., et al. 2021. Differential regulation of glycinergic and GABAergic nanocolumns at mixed inhibitory synapses. EMBO Reports, e52154.
31665629	1:200	Moretto, E., et al. 2019. TSPAN5 Enriched Microdomains Provide a Platform for Dendritic Spine Maturation through Neuroligin-1 Clustering. Cell Reports, 1130-1146.e8.
28262662	1:300	Zapata, J., et al. 2017. Epilepsy and intellectual disability linked protein Shrm4 interaction with GABABRs shapes inhibitory neurotransmission. Nature Communications, 14536.
22927941	1:500	, et al. 2012. Different subtypes of GABA-A receptors are expressed in human, mouse and rat T lymphocytes.. PLoS One, e42959.
20417281	1:100 (supe)	Smith, K.R., et al. 2010. Identification and characterisation of a Maf1/Macoco protein complex that interacts with GABAA receptors in neurons.. Molecular and Cellular Neuroscience, 330-341.

Immunohistochemistry: Mouse
PMID	Dilution	Publication
26179122	1:1000	He, Q., et al. 2015. Interneuron- and GABA(A) receptor-specific inhibitory synaptic plasticity in cerebellar Purkinje cells.. Nature Communications, 7364.
22351636	1:1000	Sumegi, M., et al. 2012. Virus-mediated swapping of zolpidem-insensitive with zolpidem-sensitive GABA(A) receptors in cortical pyramidal cells.. The Journal of Physiology, 1517-1534.
21482824	1:500	Jo, Y.H., et al. 2011. Cross-talk between P2X4 and gamma-aminobutyric acid, type A receptors determines synaptic efficacy at a central synapse.. The Journal of Biological Chemistry, 19993-20004.

Immunohistochemistry: Rat
PMID	Dilution	Publication
27537197	1:1000	Kerti-Szigeti, K., et al. 2016. Similar GABAA receptor subunit composition in somatic and axon initial segment synapses of hippocampal pyramidal cells. Elife, e18426.
24477962	1:5 (supe)	King, A.N., et al. 2014. A unique ion channel clustering domain on the axon initial segment of mammalian neurons. . Journal of Comparative Neurology, 2594-2608.
22539847	1:2000	, et al. 2012. Setting the time course of inhibitory synaptic currents by mixing multiple GABA(A) receptor α subunit isoforms.. Journal of Neuroscience, 5853-5867.
22098631	1:800	Kerti, K., et al. 2012. Unique somato-dendritic distribution pattern of Kv4.2 channels on hippocampal CA1 pyramidal cells.. European Journal of Neuroscience, 66-75.
19815531	not listed	Arancibia-Cárcamo, I.L., et al. 2009. Ubiquitin-dependent lysosomal targeting of GABA(A) receptors regulates neuronal inhibition.. PNAS: USA, 17552-17557.

Immunoprecipitation: Rat
PMID	Dilution	Publication
24089494	2ug	Pribiag, H., et al. 2014. TNF-α downregulates inhibitory neurotransmission through protein phosphatase 1-dependent trafficking of GABA(A) receptors.. Journal of Neuroscience, 15879-15893.

Western Blot: Human
PMID	Dilution	Publication
39469630	1:1000	Bundy, J, et al. 2024. GABA Type A receptors expressed in triple negative breast cancer cells mediate chloride ion flux. Frontiers in Pharmacology, 1449256.
22303015	4ug/ml	Gurba, K.N., et al. 2012. GABRB3 mutation, G32R, associated with childhood absence epilepsy alters α1β3γ2L γ-aminobutyric acid type A (GABAA) receptor expression and channel gating.. The Journal of Biological Chemistry, 12083.

Western Blot: Mouse
PMID	Dilution	Publication
39357522	1:500	Wang, G, et al. 2024. The TMEM132B-GABAA receptor complex controls alcohol actions in the brain. Cell, .
39294503	1:1000	Welle, TM, et al. 2024. miRNA-mediated control of gephyrin synthesis drives sustained inhibitory synaptic plasticity. EMBO Reports, .
34936876	1:5000	Garcia, J.D., et al. 2021. Stepwise disassembly of GABAergic synapses during pathogenic excitotoxicity. Cell Reports, 110142.
33147470	not listed	Rudolph, S., et al. 2020. ArticleCerebellum-Specific Deletion of the GABAAReceptordSubunit Leads to Sex-Specific Disruptionof Behavior. Cell Reports, 108338.
30791981	not listed	Fu, Y.L., et al. 2019. LMAN1 (ERGIC-53) promotes trafficking of neuroreceptors. Biochemical and Biophysical Research Communications, 356-362.
28154140	1 : 500	Sterky, F.H., et al. 2017. Carbonic anhydrase-related protein CA10 is an evolutionarily conserved pan-neurexin ligand.. PNAS: USA, E1253-E1262.
27044742	1:1000	Nakamura, Y., et al. 2016. Proteomic Characterization of Inhibitory Synapses Using a Novel pHluorin-tagged γ-Aminobutyric Acid Receptor, Type A (GABAA), α2 Subunit Knock-in Mouse. Journal of Biological Chemistry, 12394-12407.
24153177	not listed	Han, K., et al. 2014. SHANK3 overexpression causes manic-like behaviour with unique pharmacogenetic properties.. Nature, 72-77.
23744069	1:100	Zhou, C., et al. 2013. Altered cortical GABAA receptor composition, physiology, and endocytosis in a mouse model of a human genetic absence epilepsy syndrome.. The Journal of Biological Chemistry, 21458-21472.

Western Blot: Rat
PMID	Dilution	Publication
39918645	1:500	Sobierajski, E, et al. 2025. Expression of synaptic proteins and development of dendritic spines in fetal and postnatal neocortex of the pig, the European wild boar Sus scrofa. Brain Structure & Function, 38.
32579917	1:1000	Rajgor, D., et al. 2020. Local miRNA-dependent translational control of GABAAR synthesis during inhibitory long-term potentiation. Cell reports, 31(12), p.107785.. Cell reports, 107785.
25310985	not listed	Piguel, N.H., et al. 2014. Scribble1/AP2 complex coordinates NMDA receptor endocytic recycling. Cell Reports, 712-727.
24089494	1:500	Pribiag, H., et al. 2014. TNF-α downregulates inhibitory neurotransmission through protein phosphatase 1-dependent trafficking of GABA(A) receptors.. Journal of Neuroscience, 15879-15893.
20417281	1:10 (supe)	Smith, K.R., et al. 2010. Identification and characterisation of a Maf1/Macoco protein complex that interacts with GABAA receptors in neurons.. Molecular and Cellular Neuroscience, 330-341.

Additional Publications: Unspecified
PMID	Publication
26732589	Mele, M., et al. 2017. Downregulation of GABAA Receptor Recycling Mediated by HAP1 Contributes to Neuronal Death in In Vitro Brain Ischemia.. Molecular Neurobiology, 45-57.
25510230	Babateen, O., et al. 2015. Etomidate, propofol and diazepam potentiate GABA-evoked GABAA currents in a cell line derived from human glioblastoma.. European Journal of Pharmacology, 101-107.
25402556	Marty, V.N., et al. 2015. Radiation-induced alterations in synaptic neurotransmission of dentate granule cells depend on the dose and species of charged particles.. Radiation Research, 653-665.
24513087	Mele, M., et al. 2014. GABA(A) receptor dephosphorylation followed by internalization is coupled to neuronal death in in vitro ischemia.. Neurobiology of Disease, 220-232.
20980909	Fagerlund, M.J., et al. 2010. The human carotid body: expression of oxygen sensing and signaling genes of relevance for anesthesia.. Anesthesiology, 1270-1279.
20470805	Adusei, D.C., et al. 2010. Early developmental alterations in GABAergic protein expression in fragile X knockout mice.. Neuropharmacology, 167-171.

Anti-GABA-A-R, Beta3 Antibody

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SKU: 75-149

Product Details

Product Specific References for Applications and Species

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