| Short Description : | Rabbit polyclonal EIF3A (1-250) antibody for use in WB, IF, ICC and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | WB/IF/ICC/ELISA |
| Host: | Rabbit |
| Reactivity: | Human/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | Lot specific |
| Purification: | Affinity purification |
| Dilution Range: | WB:1:1000-1:5000IF/ICC:1:50-1:100ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | |
| Gene ID: | |
| Uniprot ID: | |
| Immunogen Region: | 1-250 |
| Immunogen Sequence: | MPAYFQRPENALKRANEFLE VGKKQPALDVLYDVMKSKKH RTWQKIHEPIMLKYLELCVD LRKSHLAKEGLYQYKNICQQ VNIKSLEDVVRAYLKMAEEK TEAAKEESQQMVLDIEDLDN IQTPESVLLSAVSGEDTQDR TDRLLLTPWVKFLWESYRQC LDLLRNNSRVERLYHDIAQQ AFKFCLQYTRKAEFRKLCDN LRMHLSQIQRHHNQSTAINL NNPESQSMHLETRLVQLDS |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human EIF3A (NP_003741.1). |
| Post Translational Modifications | Phosphorylated. Phosphorylation is enhanced upon serum stimulation. |
| Function | RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression. (Microbial infection) Essential for the initiation of translation on type-1 viral ribosomal entry sites (IRESs), like for HCV, PV, EV71 or BEV translation. (Microbial infection) In case of FCV infection, plays a role in the ribosomal termination-reinitiation event leading to the translation of VP2. |
| Protein Name | Eukaryotic Translation Initiation Factor 3 Subunit AEif3aEukaryotic Translation Initiation Factor 3 Subunit 10Eif-3-ThetaEif3 P167Eif3 P180Eif3 P185 |
| Database Links | |
| Cellular Localisation | Cytoplasm |
| Alternative Antibody Names | Anti-Eukaryotic Translation Initiation Factor 3 Subunit A antibodyAnti-Eif3a antibodyAnti-Eukaryotic Translation Initiation Factor 3 Subunit 10 antibodyAnti-Eif-3-Theta antibodyAnti-Eif3 P167 antibodyAnti-Eif3 P180 antibodyAnti-Eif3 P185 antibodyAnti-EIF3A antibodyAnti-EIF3S10 antibodyAnti-KIAA0139 antibody |
Information sourced from Uniprot.org