Mouse Anti-Doublesex Antibody
Product Description
Specifications
| Size | 20 µg, 100 µg, 200 µg, 500 µg |
| Species | Insect |
| Accession Number | AAA17840 |
| Clonality | Monoclonal |
| Clone | 2E11.C112 |
| Immunogen | Immunogen was recombinant protein derived from Drosophila Doublesex (aa35-93). This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with the immunogen. The IgG fraction of tissue culture supernatant was purified by Protein G/A affinity chromatography. |
| Recommended Applications | ELISA (recommended work dilution= 1: 1, 000-5, 000), Western Blotting (recommended work dilution= 1: 1, 000-2, 000) |
| Reconstitution | Supplied as lyophilized and purified antibody originally containing PBS, without Preservative Stabilizers, such as Sodium Azide. The final concentration is indicated on the shipping vial. The antibody is stable for at least years from the data of receipt when stored at -20°C to -70°C. Reconstituted antibody (recommended with H20 1 mg/ml) can also be aliquoted and stored frozen at -20°C to -70°C in a manual defrost freezer for months without detectable loss activity. Upon reconstitution, the antibody can be stored at 4°C for 1 month. Please avoid freeze-thaw cycles. |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
The doublesex (dsx) gene regulates somatic sexual differentiation in both sexes in D. melanogaster. Two functional products are encoded by dsx: one product is expressed in females and represses male differentiation, and the other is expressed in males and represses female differentiation. The protein functions are needed to further investigate.
Specificity
This antibody was selected for its ability to detect Drosophila Doublesex (aa35-93). In ELISA and Western Blot, this antibody showed specifically binding to recombinant protein from Drosophila Doublesex and there was no cross-reactivity with tested proteins from Drosophila Doublesex.
Storage
Store at -20°C, if not intended for use within a month, keep it at 4°C and minimize freezing and thawing when use.
References
- Baker, B. S., and Belote, J. M. (1983). Sex determination and dosage compensation in Drosophila melanogasfer. Annu. Rev. Genet. 77,345-393.
- Oliver, B., Perrimon, N.. and Mahowald, A. l? (1988). Genetic evidence that the saris fille locus is involved in Drosophila sex determination.Genetics 720, 159-171.
Expiration:
12 months from the date of shipment when stored properly.



