Anti-DNMT1 Antibody

Referência CQA0193-100ul

Tamanho : 100ul

Marca : Cohesion Biosciences

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  • Western blot analysis of DNMT1 expression in Jurkat (A), COS7 (B), HCT116 (C), NTERA2 (D) whole cell lysates. (Predicted band size: 183 kD; Observed band size: 183 kD kD)
  • Immunohistochemical analysis of DNMT1 staining in human rectum cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Product Name:Anti-DNMT1 Antibody
Cat No:CQA0193
Source:Mouse
Reactivity:H, Mk
Applications:WB, IH
*Application Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
*Species Reactivity Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
Description:Mouse monoclonal to DNMT1
Immunogen:Recombinant fusion protein of human DNMT1 expressed in E. Coli
Purification:This antibody is purified through a protein G column.
Specificity:Recognizes endogenous levels of DNMT1 protein
Clonality:Monoclonal
Form:Mouse IgG1. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Dilution:WB (1/500 - 1/1000), IH (1/100 - 1/500)
Gene Symbol:DNMT1
Alternative Names:AIM; CXXC9; DNMT; DNA (cytosine-5)-methyltransferase 1; Dnmt1; CXXC-type zinc finger protein 9; DNA methyltransferase HsaI; DNA MTase HsaI; M.HsaI; MCMT
Entrez Gene (Human): 1786;
SwissProt (Human): P26358;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.