RM181 specifically reacts to Histone H3 dimethylated Western Blot of recombinant histone H3.3 (1) ChIP performed on HeLa cells using H3K79me2 antibody Immunohistochemistry staining of HepG2 cells using
Product Details Product Name Anti-Dimethyl-Histone H3 (Lys79) Rabbit Monoclonal Antibody, Clone RM181 Product Description Rabbit monoclonal to Dimethylated Histone H3 Lysine 79, H3K79me2, Histone H3 (dimethyl K79) Catalog No. 31-1065-00 Clone Name RM181 Product Data Sheet Anti-Dimethyl-Histone H3 (Lys79) Rabbit Monoclonal Antibody Data Sheet RM181 Specificity RM181 reacts to Histone H3 dimethylated at Lysine 79 (K79me2). ). Very slightly cross-reacts with monomethylated Lysine 79 (K79me1) at high concentration. No cross reactivity with non-modified Lysine 79 (K79 ctrl), trimethylated Lysine 79 (K79me3), or other methylations in Histone H3. Immunogen A dimethyl-peptide corresponding to Dimethyl-Histone H3 (Lys79). Application Western Blot (WB)
Chromatin IP (ChIP)
Immunohistochemistry (IHC)
ELISA
Multiplex
Species Reactivity Human
Vertebrates Conjugate None Intended Use Research Use Only . Properties Form Liquid Storage Instructions Store at -20.0°C Stability Stable for 1 Year at -20.0°C from date of receipt Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide Volume/Size 100 µg Concentration 1.0 mg/mL Purity Protein A affinity purified from an animal origin–free culture supernatant Clone Type Monoclonal Isotype Rabbit IgG Usage Western Blot: 0.25 µg/mL – 1 µg/mL;
IHC: 0.1 µg/mL – 1 µg/mL;
ChIP: 2 µg/mL - 10 µg.mL;
ELISA: 0.2 µg/mL - 1 µg/mL;
Multiplex: 0.1 µg/mL – 0.5 µg/mL. Keywords . Product Images
at Lysine 79 (K79me2). Very slightly cross reactivity
with monomethylated Lysine 14 (K14me1), and no
cross reactivity with non-modified Lysine 79 (K79 ctrl),
trimethylated Lysine 79 (K79me3), or other methylations
in Histone H3.
and acid extracts of HeLa cells (2), using RM181
at 0.25 µg/mL, showed a band of histone H3
dimethylated at Lysine 79 (K79me2) in HeLa cells.
(RM181, 5 µg). Real-time PCR was performed using primers
specific to the gene indicated.
Anti-Dimethyl-Histone H3 (Lys79) antibody, Clone RM181.
Anti-Dimethyl Histone H3 (Lys79) rabbit monoclonal antibody [RM181]
![Anti-Dimethyl Histone H3 (Lys79) rabbit monoclonal antibody [RM181]](https://www.quimigen.pt/upload/-img-lapin1.png)
Referência 31-1065-00
Tamanho : 100ug
Marca : RevMab Biosciences
Contactar o distribuidor local :
Telefone : +1 850 650 7790
Rabbit monoclonal to Dimethylated Histone H3 Lysine 79, H3K79me2, Histone H3 (dimethyl K79)
25 µg Trial Size, 100 µg Full Size
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