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> Anti-CDC2 (Phospho-T161) Antibody

Anti-CDC2 (Phospho-T161) Antibody

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Referência CPA1189-30ul

Tamanho : 30ul

Marca : Cohesion Biosciences

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Telefone : +1 850 650 7790

  • Western blot analysis of CDC2 (Phospho-T161) expression in HepG2 (A) whole cell lysates. (Predicted band size: 34 kD; Observed band size: 34 kD)
  • Immunohistochemical analysis of CDC2 (Phospho-T161) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  • Direct ELISA antibody dose-response curve using Anti-CDC2 (Phospho-T161) Antibody. Antigen (Phosphopeptide and non-phosphopeptide) concentration is 5 ug/ml. Goat Anti-Rabbit IgG (H&L) - HRP was used as the secondary antibody, and signal was developed by TMB substrate.
Product Name:Anti-CDC2 (Phospho-T161) Antibody
Cat No:CPA1189
Source:Rabbit
Reactivity:H, M, R, B, C, Mk, Z
Applications:WB, IH
*Application Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
*Species Reactivity Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
Description：Rabbit polyclonal antibody to CDC2 (Phospho-T161)
Immunogen：KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding T161 of human CDC2 protein. The exact sequence is proprietary.
Purification：The antibody was purified by immunogen affinity chromatography.
Specificity：Recognizes endogenous levels of CDC2 protein only when phosphorylated at T161.
Clonality：Polyclonal
Form：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Dilution：WB (1/500 - 1/1000), IH (1/50 - 1/100)
Gene Symbol：CDK1; CDK2
Alternative Names：CDC2; CDC28A; CDKN1; P34CDC2; Cyclin-dependent kinase 1; CDK1; Cell division control protein 2 homolog; Cell division protein kinase 1; p34 protein kinase
Entrez Gene (Human)： 983;
SwissProt (Human)： P06493;
Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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