Anti-CD23 Antibody-APC labled
Referência CFB8508-100
Tamanho : 100Assays
Marca : Cohesion Biosciences
| Product Name: | Anti-CD23 Antibody-APC labled |
| Cat No: | CFB8508 |
| Source: | Mouse |
| Reactivity: | H |
| Applications: | IF, FC |
| *Application Key: | |
| H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish | |
| *Species Reactivity Key: | |
| E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference | |
Description:Mouse monoclonal antibody APC labled to CD23Immunogen:Native purified human CD23.Purification:The antibody was purified by affinity chromatography.Specificity:Recognizes human CD23Clonality:Monoclonal (clone: EBVCS-5)Conjugation:APCForm:Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 0.2% BSA, and 0.02% sodium azide.Dilution:10 μl / assayGene Symbol:FCER2Alternative Names:CD23A; CLEC4J; FCE2; IGEBF; Low affinity immunoglobulin epsilon Fc receptor; BLAST-2; C-type lectin domain family 4 member J; Fc-epsilon-RII; Immunoglobulin E-binding factor; Lymphocyte IgE receptor; CD antigen CD23) Entrez Gene (Human): 2208; SwissProt (Human): P06734; Directions for Use:1. Take 100 μl peripheral blood anticoagulated by EDTA and add to the bottom of 5 ml tube. 2. Add 10 μl labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 ml RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 ml PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured). Storage/Stability:Shipped and store at 4°C for one year. Do not freeze.

