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> Anti-Calbindin/CALB1 Antibody Picoband®

Anti-Calbindin/CALB1 Antibody Picoband®

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Referência A03047

Tamanho : 100ug/vial

Marca : Boster Bio

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Telefone : +1 850 650 7790

A03047 Calbindin primary antibodies FC testing 5
a03047 calbindin primary antibodies if testing 6
a03047 calbindin primary antibodies if testing 7
a03047 calb1 primary antibodies ihc testing 5
A03047 Calbindin primary antibodies IHC testing 2
A03047 Calbindin primary antibodies IHC testing 3
A03047 Calbindin primary antibodies IHC testing 4
a03047 calbindin primary antibodies wb testing 1

Tested Applications

CALB1 antibody was positively detected by WB in:

rat brain tissue, rat kidney tissue, mouse brain tissue, mouse kidney tissue

Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane

CALB1 antibody was positively detected by IHC in:

rat kidney tissue, mouse brain tissue, mouse kidney tissue

Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.

CALB1 antibody was positively detected by IF in:

rat cerebellum tissue, rat cerebellum tissue

CALB1 antibody was positively detected by FC in:

A431 cell

Recommend Dilution

Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Human, Mouse, Rat
Immunofluorescence
5 μg/ml
Rat
Flow Cytometry (Fixed)
1-3μg/1x106 cells
Human
ELISA
0.1-0.5μg/ml
-

* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.

Note: For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.

Product Information

Product Name
Anti-Calbindin/CALB1 Antibody Picoband®
View all CALB1 Antibodies
Form
Lyophilized
Uniprot
P05937
Gene ID
793
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human Calbindin recombinant protein (Position: A2-N261).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
30 kDa
Calculated Molecular Weight

30.0 kDa

Background of CALB1
Calbindin is a calcium-binding protein belonging to the troponin C superfamily. And it mapped to 8q21.3. Calretinin is expressed in central and peripheral nervous system and in many normal and pathological tissues. The rat and human calretinin exhibit 98% sequence homology and 91% homology to many other species. Two calcium binding proteins, calbindin and calretinin, have been reported to be expressed in abundance in Purkinje cells and other cell types in the cerebellum.
Cite This Product
Anti-Calbindin/CALB1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03047)

Protocols

Western Blot protocol for CALB1 antibody
Review Protocol
Immunohistochemistry (IHC) protocol for CALB1 antibody
Review Protocol
Immunofluorescence (IF) protocol for CALB1 antibody
Review Protocol
Flow Cytometry (FC) protocol for CALB1 antibody
Review Protocol

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