General Information
| Short Description | Mouse monoclonal anti-Blood Group A (CD173) for use in AGG, FC, IF and IHC in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | AGG/FC/IF/IHC |
| Host | Mouse |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | HE-10 |
| Isotype | IgMk |
| Conjugation | Unconjugated |
| Concentration | Can be provided as 100 µg/mL, 500 µg/mL or 1mg/mL. |
| Purification | Affinity purified from tissue culture. |
| Dilution Range | Agglutination. Flow cytometry (0, 5–1, 0 µg/million cells in 0, 1 ml). Immunofluorescence (0, 5–1 µg/ml). Immunohistology (formalin-fixed: 1-2 µg/ml for 30 min. at RT; requires boiling tissue sections in 10mM citrate buffer, pH 6, 0, for 10-20 min fo |
| Formulation | PBS with 0.02% Sodium Azide. |
| Storage Instruction | Store for up to 1 year at 2-8°C upon receipt. |
Target Information
| Immunogen | A BALB/c mouse was immunized with a mixture of erythrocytes of blood group A1 and a glycoprotein fraction isolated from the saliva of secretors with blood group A of human origin. Fusion partner: P3-X63-Ag8.653. |
Additional Info
| Background | Agglutinates erythrocytes of group A, and is excellent as a tumour marker in patients ofblood group B and 0. It does not agglutinate erythrocytes of group B and 0. Study with specificoligosaccharides showed that the antibody HE-10 reacts with A and H antigens with chain types 3 and4 and it does not react with A disaccharide, A trisaccharide, A type 1, A type 2, ALeb. The antibody HE-10 does not react with normal tissue sections of donors with blood group B and 0 but it reactsspecifically with malignant tissues.Immunogen: Mixture of erythrocytes of group A1 and glycoprotein fraction isolated from saliva ofsecretors with blood group A. |
Information sourced from Uniprot.org