Anti-ASCL1/Mash1 Antibody

Referência A03023

Tamanho : 100ul

Marca : Boster Bio

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Telefone : +1 850 650 7790

Tested Applications

ASCL1 antibody was positively detected by WB in:

human K562 whole cell, human U251 whole cell, human PC-3 whole cell, human NCL-H460 whole cell, rat brain tissue, rat PC-12 whole cell, mouse brain tissue, mouse Neuro-2a whole cell

Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane

ASCL1 antibody was positively detected by IHC in:

human lung cancer tissue

Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.

ASCL1 antibody was positively detected by FC in:

K562 cells

Recommend Dilution

Application
Dilution
Species
Western blot
0.25-0.5 μg/ml
Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section)
2-5 μg/ml
Human
Flow Cytometry (Fixed)
1-3 μg/1x106 cells
Human

* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.

Note: For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.

Product Information

Product Name
Anti-ASCL1 Antibody Picoband®
View all ASCL1 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human ASCL1, identical to the related mouse and rat sequences.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
30 kDa
Calculated Molecular Weight

25.5 kDa

Background of ASCL1
Achaete-scute homolog 1?is a?protein?that in humans is encoded by the?ASCL1?gene. This gene encodes a member of the basic helix-loop-helix (BHLH) family of transcription factors. The protein activates transcription by binding to the E box (5'-CANNTG-3'). Dimerization with other BHLH proteins is required for efficient DNA binding. This protein plays a role in the neuronal commitment and differentiation and in the generation of olfactory and autonomic neurons. Mutations in this gene may contribute to the congenital central hypoventilation syndrome (CCHS) phenotype in rare cases.
Cite This Product
Anti-ASCL1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03023-2)

Protocols

Western Blot protocol for ASCL1 antibody
Immunohistochemistry (IHC) protocol for ASCL1 antibody
Flow Cytometry (FC) protocol for ASCL1 antibody

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