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AM4-65

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Referência 70039

Tamanho : 1MG

Marca : Biotium

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AM4-65

AM4-65 is a fixable red fluorescent nerve terminal probe. The dye is spectrally identical to SynaptoRed™ C2 (equivalent to FM®4-64), but it has an additional amine group at the hydrophilic end of the molecule, making the dye fixable with formaldehyde or gluteraldehyde.

Size
Catalog #
Qty
1 mg
#70039
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AM4-65
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Product Description

AM4-65 is a fixable red fluorescent nerve terminal probe. The dye is spectrally identical to SynaptoRed™ C2 (equivalent to FM®4-64), but it has an additional amine group at the hydrophilic end of the molecule, making the dye fixable with formaldehyde or gluteraldehyde. The long emission wavelength makes the dye suitable to be used with GFP in two-color imaging.

  • λEx/λEm (membranes) = ~510/750 nm*
  • Dark purple solid soluble in water
  • MW: 844.85

* Excitation/emission settings of 515/640 nm have been used for detection of yeast vacuole staining with SynaptoRed™ C2 (FM®4-64), and should be applicable to AM4-65.

Nerve Terminal Dyes and Kits

ProductCatalog No.Features
SynaptoGreen™ Dyes
(Ex/Em ~480/660 nm in membranes)		70020... 70053View full list of SynaptoGreen™ Dyes
SynaptoRed Dyes™
(Ex/Em ~510/750 nm in membranes)		70021... 70050View full list of SynaptoRed™ Dyes
Background Reducers
ADVASEP-770029Sulfonated cyclodextrin that aids in removal of free dye during washes
SCAS70037Quenches extracellular fluorescences with fewer wash steps than ADVASEP-7
Sulforhodamine 10180101Red fluorescent dye that quenches extracellular fluorescence of SynaptoGreen™ dyes
Nerve Terminal Staining Kits
Nerve Terminal Staining Kit I70030Includes SynaptoGreen™ C4 and ADVASEP-7
Nerve Terminal Staining Kit II (A)70031Includes AM1-43 and ADVASEP-7
Nerve Terminal Staining Kit II (B)70031-1Includes AM1-43 and SCAS
Nerve Terminal Staining Kit III70032Includes SynaptoGreen™ C4 and Sulforhodamine 101
Nerve Terminal Staining Kit V70034Includes SynaptoRed™ C2 and ADVASEP-7
m is the number of carbons in the lipophilic tail and n is the number of double bonds linking the two aromatic rings in the dye.
**The positively-charged end of SynaptoRed C2M is a trimethylammonium group.
FM is a registered trademark of Thermo Fisher Scientific.
Technology
Technology Icon
Membrane Potential & Nerve Terminal Dyes
Technology Icon
Neuronal Probes and Tools

Product Attributes

Size
1 mg
Probe cellular localization
Membrane/vesicular
For live or fixed cells
For live/intact cells
Assay type/options
Real-time imaging
Fixation options
Fix after staining (formaldehyde), Permeabilize after staining
Colors
Far-red
Excitation/Emission
510/750 nm (see product description)

Documents, Protocols, SDS and COA

COA
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Citations

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Cond.
Price Bef. VAT
70024
AM1-43
 1MG 
70025
AM4-64
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70050
AM4-66
 1MG