Anti-Alcohol Dehydrogenase (Yeast) (RABBIT) Antibody Peroxidase Conjugated, 100 ug
Size:
100 ug
Conjugation:
HRP
Western Blot of Rabbit anti-Alcohol Dehydrogenase (Yeast) Antibody Peroxidase Conjugated. Lane 1: Alcohol Dehydrogenase (yeast). Load: 50 ng per lane. Primary antibody: Rabbit anti-Alcohol Dehydrogenase (Yeast) Antibody Peroxidase Conjugated at 1:1,000 overnight at 4°C. Secondary antibody: n/a. Block: MB-070 for 30 min at RT. Predicted/Observed size: 37 kDa, 45 kDa for Alcohol Dehydrogenase (yeast).
Alcohol Dehydrogenase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Alcohol Dehydrogenase Yeast. Cross reactivity against Alcohol Dehydrogenase from other sources is unknown.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free Preservative: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
Reconstitution Method
Restore with deionized water (or equivalent) - Reconstitution Volume: 100 µL
Concentration
1.0 mg/mL - lot specific
Purification
Multi-step process including delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
Conjugation
HRP
Storage
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Stability
Expiration date is one (1) year from date of receipt.
Alcohol Dehydrogenases (ADH) are a group of dehydrogenase enzymes that occur in many organisms and facilitate the interconversion between alcohols and aldehydes or ketones with the reduction of nicotinamide adenine dinucleotide (NAD+ to NADH). In humans and many other animals, they serve to break down alcohols that otherwise are toxic, and they also participate in generation of useful aldehyde, ketone, or alcohol groups during biosynthesis of various metabolites.
Anti-Alcohol Dehydrogenase Peroxidase Conjugated Antibody has been tested by ELISA and western blot and is suitable in immunohistochemistry. Optimal titers are to be optimized by researchers.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
