ADAM10 ELISA Kit (Mouse)
Referência OKEH06827
Tamanho : 96Wells
Marca : Aviva Systems Biology
Contactar o distribuidor local :
Telefone : +1 850 650 7790
ADAM10 ELISA Kit (Mouse) (OKEH06827)
| Datasheets/Manuals | Printable datasheet for ADAM10 ELISA Kit (Mouse) (OKEH06827) |
|---|---|
| COA Datasheet | Printable COA datasheet for OKEH06827 |
| Predicted Species Reactivity | Mouse|Mus musculus | ||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Application | ELISA-Sandwich | ||||||||||||||||||||||
| ELISA Kit Detection Method | Colorimetric, OD 450 nm | ||||||||||||||||||||||
| ELISA Kit Duration | ~3 Hours | ||||||||||||||||||||||
| ELISA Kit Principle | Aviva Systems Biology Adam10 ELISA Kit (Mouse) (OKEH06827) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Adam10 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Adam10 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Adam10 captured in the well. | ||||||||||||||||||||||
| ELISA Kit Range | 0.156-10ng/mL | ||||||||||||||||||||||
| ELISA Kit Reproducibility | Intra-Assay Reproducibility CV% < 7.1% (n=20)< br>Inter-Assay Reproducibility CV% < 9.2% (n=24) | ||||||||||||||||||||||
| ELISA Kit Component |
| ||||||||||||||||||||||
| Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
| Sample Type | Serum, plasma and other biological fluids | ||||||||||||||||||||||
| Sensitivity | 0.081 ng/mL | ||||||||||||||||||||||
| Specificity | Natural and recombinant Mouse Disintegrin and metalloproteinase domain-containing protein 10 | ||||||||||||||||||||||
| Assay Info | Assay Methodology: Quantitative Sandwich ELISA |
| Gene Symbol | Adam10 |
|---|---|
| Gene Full Name | a disintegrin and metallopeptidase domain 10 |
| Alias Symbols | 1700031C13Rik, a disintegrin and metalloprotease domain (ADAM) 10, disintegrin and metalloproteinase domain-containing protein 10, kuz, kuzb, kuzbanian, kuzbanian protein homolog, MADM, mammalian disintegrin-metalloprotease. |
| NCBI Gene Id | 11487 |
| Protein Name | Disintegrin and metalloproteinase domain-containing protein 10 |
| Description of Target | Cleaves the membrane-bound precursor of TNF-alpha to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface (By similarity). Responsible for the proteolytic release of several other cell-surface proteins, including heparin-binding epidermal growth-like factor, ephrin-A2, CD44, CDH2 and for constitutive and regulated alpha-secretase cleavage of amyloid precursor protein (APP) at '687-Lys-|-Leu-688' (By similarity). Contributes to the normal cleavage of the cellular prion protein (By similarity). Involved in the cleavage of the adhesion molecule L1 at the cell surface and in released membrane vesicles, suggesting a vesicle-based protease activity (By similarity). Controls also the proteolytic processing of Notch and mediates lateral inhibition during neurogenesis (PubMed:9244301). Responsible for the FasL ectodomain shedding and for the generation of the remnant ADAM10-processed FasL (FasL APL) transmembrane form (By similarity). Also cleaves the ectodomain of the integral membrane proteins CORIN and ITM2B (By similarity). May regulate the EFNA5-EPHA3 signaling (By similarity). |
| Uniprot ID | O35598 |
| Protein Accession # | NP_031425 |
| Nucleotide Accession # | NM_007399 |
