Sample Type:|Serum, plasma, tissue homogenates and other biological fluids.||Intended Use:|Competitive ELISA, Coated with Antigen||Sensitivity:|<7.8pg/ml||Range:|15.6-1000pg/ml||Specificity:|Rat||Test Principle:|This BioAssay™ ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with 4-HNE. During the reaction, 4-HNE in the sample or standard competes with a fixed amount of 4-HNE on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to 4-HNE. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin (SABC) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm. The concentration of 4-HNE in the samples is then determined by comparing the O.D. of the samples to the standard curve.||Kit Components:|Microtiter Strips, 1x48 wells or 1x96 wells |Standard, 1x1 vial or 2x1 vials|Sample/Standard Dilution Buffer, 1x10ml or 1x20ml|Antibody (Biotin) (Concentrated), 1x30ul or 1x60ul|Antibody Dilution Buffer, 1x5ml or 1x10ml|Streptavidin (HRP) (SABC), 1x60ul or 1x120ul|SABC Dilution Buffer, 1x5ml or 1x10ml|TMB Substrate, 1x5ml or 1x10ml|Stop Solution, 1x5ml or 1x10ml|Wash Buffer, 25X, 1x15ml or 1x30ml||Storage and Stability:|Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.