OKCD02265 | 1-Desamino 8D Arginine Vasopressin ELISA Kit Quimigen

Technical Documents

Datasheets/Manuals	Printable datasheet for 1-Desamino 8D Arginine Vasopressin ELISA Kit (OKCD02265)

Product Info

Predicted Species Reactivity

All Species

Application

ELISA-Comp

ELISA Kit Detection Method

Colorimetric

ELISA Kit Duration

2h

ELISA Kit Linearity

Matrix	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
serum(n=5)	83-94%	99-105%	96-103%	83-101%
EDTA plasma(n=5)	83-90%	82-89%	78-99%	96-103%
heparin plasma(n=5)	88-95%	80-91%	78-102%	78-89%

ELISA Kit Principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to 1-Desamino 8D Arginine Vasopressin (DDAVP) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled 1-Desamino 8D Arginine Vasopressin (DDAVP) and unlabeled 1-Desamino 8D Arginine Vasopressin (DDAVP) (Standards or samples) with the pre-coated antibody specific to 1-Desamino 8D Arginine Vasopressin (DDAVP). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of 1-Desamino 8D Arginine Vasopressin (DDAVP) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of 1-Desamino 8D Arginine Vasopressin (DDAVP) in the sample.

ELISA Kit Range

246.9-20,000pg/mL

ELISA Kit Recovery

Matrix	Recovery Range (%)	Average (%)
serum(n=5)	84-105	102
EDTA plasma(n=5)	90-101	93
heparin plasma(n=5)	78-96	83

ELISA Kit Reproducibility

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level 1-Desamino 8D Arginine Vasopressin (DDAVP) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level 1-Desamino 8D Arginine Vasopressin (DDAVP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

ELISA Kit Component

Component	Amount
Anti-1-Desamino 8D Arginine Vasopressin Microplate	96 Wells (12 x 8 Well Strips)
1-Desamino 8D Arginine Vasopressin Lyophilized Standard	2
100X 1-Desamino 8D Arginine Vasopressin Biotin Complex	120 uL
100X Avidin/HRP Conjugate	120 uL
Standard Diluent	1 x 20 mL
Biotin Complex Diluent	1 x 12 mL
Avidin/HRP Conjugate Diluent	1 x 12 mL
30X Wash Buffer	1 x 20 mL
TMB Substrate	1 x 9 mL
Stop Solution	1 x 6 mL

Additional Information

CAS: 16679-58-6
PubChem: 5311065

Reconstitution and Storage

2°C to 8°C|-20°C

Sample Type

Serum, plasma and other biological fluids.

Sensitivity

39.5 pg/mL

Assay Info

Assay Methodology: Competitive Inhibition Immunoassay

Target Info

Gene Symbol	DDAVP
Alias Symbols	DESMOPRESSIN; 16679-58-6; DDAVP; UNII-ENR1LLB0FP
Protein Name	1-Desamino 8D Arginine Vasopressin
Description of Target	Desmopressin is a Factor VIII Activator and Vasopressin Analog. The physiologic effect of desmopressin is by means of Increased Coagulation Factor VIII Activity and Increased Coagulation Factor VIII Concentration. The chemical classification of desmopressin is Vasopressins.

Protocols & Data

1-Desamino 8D Arginine Vasopressin ELISA Kit

Referência OKCD02265

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Marca : Aviva Systems Biology

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1-Desamino 8D Arginine Vasopressin ELISA Kit (OKCD02265)